gel red in tbe Search Results


0.5% tbe agarose gel starstain red nucleic acid dye  (GenStar Biosolutions)
90
GenStar Biosolutions 0.5% tbe agarose gel starstain red nucleic acid dye
Biochemical and functional characterizations of AcrIIA17 and AcrIIA18. (A, B) In vitro <t>DNA</t> <t>cleavage</t> assay of Cas9 in the presence of AcrIIA17 ( A ) or AcrIIA18 ( B ). The assay was performed with different orders of addition as described in the schematic (lower inset). The pre-incubated components are shown in parentheses. These gels are representative of three replicate experiments. (C, D) A <t>TBE-urea</t> denaturing gel showing AcrIIA17- ( C ) or AcrIIA18-mediated ( D ) cleavage of sgRNA. The assay was performed with different orders of addition. dCas9: dead mutant Cas9. These gels are representative of three replicate experiments. (E, F) The gel electrophoresis mobility shift assay (EMSA) on sgRNA in the presence of AcrIIA17 ( E ) or AcrIIA18 ( F ). The assay was performed with different orders of addition. These gels are representative of three replicate experiments.
0.5% Tbe Agarose Gel Starstain Red Nucleic Acid Dye, supplied by GenStar Biosolutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gel+red+in+tbe/0+5++tbe+agarose+gel+starstain+red+nucleic+acid+dye/pmc08754659-101-9-19
Average 90 stars, based on 1 article reviews
0.5% tbe agarose gel starstain red nucleic acid dye - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
12% tbe-urea page gel prestained with starstain red  (GenStar Biosolutions)
90
GenStar Biosolutions 12% tbe-urea page gel prestained with starstain red
Biochemical and functional characterizations of AcrIIA17 and AcrIIA18. (A, B) In vitro <t>DNA</t> <t>cleavage</t> assay of Cas9 in the presence of AcrIIA17 ( A ) or AcrIIA18 ( B ). The assay was performed with different orders of addition as described in the schematic (lower inset). The pre-incubated components are shown in parentheses. These gels are representative of three replicate experiments. (C, D) A <t>TBE-urea</t> denaturing gel showing AcrIIA17- ( C ) or AcrIIA18-mediated ( D ) cleavage of sgRNA. The assay was performed with different orders of addition. dCas9: dead mutant Cas9. These gels are representative of three replicate experiments. (E, F) The gel electrophoresis mobility shift assay (EMSA) on sgRNA in the presence of AcrIIA17 ( E ) or AcrIIA18 ( F ). The assay was performed with different orders of addition. These gels are representative of three replicate experiments.
12% Tbe Urea Page Gel Prestained With Starstain Red, supplied by GenStar Biosolutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gel+red+in+tbe/12++tbe+urea+page+gel+prestained+with+starstain+red/pm36302881-206-20-22
Average 90 stars, based on 1 article reviews
12% tbe-urea page gel prestained with starstain red - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

Image Search Results


Biochemical and functional characterizations of AcrIIA17 and AcrIIA18. (A, B) In vitro DNA cleavage assay of Cas9 in the presence of AcrIIA17 ( A ) or AcrIIA18 ( B ). The assay was performed with different orders of addition as described in the schematic (lower inset). The pre-incubated components are shown in parentheses. These gels are representative of three replicate experiments. (C, D) A TBE-urea denaturing gel showing AcrIIA17- ( C ) or AcrIIA18-mediated ( D ) cleavage of sgRNA. The assay was performed with different orders of addition. dCas9: dead mutant Cas9. These gels are representative of three replicate experiments. (E, F) The gel electrophoresis mobility shift assay (EMSA) on sgRNA in the presence of AcrIIA17 ( E ) or AcrIIA18 ( F ). The assay was performed with different orders of addition. These gels are representative of three replicate experiments.

Journal: Nucleic Acids Research

Article Title: Inhibition mechanisms of CRISPR-Cas9 by AcrIIA17 and AcrIIA18

doi: 10.1093/nar/gkab1197

Figure Lengend Snippet: Biochemical and functional characterizations of AcrIIA17 and AcrIIA18. (A, B) In vitro DNA cleavage assay of Cas9 in the presence of AcrIIA17 ( A ) or AcrIIA18 ( B ). The assay was performed with different orders of addition as described in the schematic (lower inset). The pre-incubated components are shown in parentheses. These gels are representative of three replicate experiments. (C, D) A TBE-urea denaturing gel showing AcrIIA17- ( C ) or AcrIIA18-mediated ( D ) cleavage of sgRNA. The assay was performed with different orders of addition. dCas9: dead mutant Cas9. These gels are representative of three replicate experiments. (E, F) The gel electrophoresis mobility shift assay (EMSA) on sgRNA in the presence of AcrIIA17 ( E ) or AcrIIA18 ( F ). The assay was performed with different orders of addition. These gels are representative of three replicate experiments.

Article Snippet: The cleavage products were separated and visualized by 0.5% TBE agarose gel pertained with StarStain Red Nucleic Acid Dye (GenStar).

Techniques: Functional Assay, In Vitro, DNA Cleavage Assay, Incubation, Mutagenesis, Nucleic Acid Electrophoresis, Mobility Shift

The N-terminal β-hairpin in NTD is critical for AcrIIA18 function. ( A ) The charged residues of AcrIIA18 selected for mutagenesis are shown in pink sphere representations ( B ) In vitro DNA cleavage assay in the presence of the wild-type or mutated AcrIIA18. This gel is representative of three replicate experiments. ( C ) Representative TBE-urea denaturing gels showing the sgRNA cleavage by wild-type and mutated AcrIIA18 in the presence of Cas9. This gel is representative of three replicate experiments. ( D ) The V-shaped groove of AcrIIA18. Charged residues Lys15, Arg19 and Arg52 are located in the V-shaped groove. Tyr17 stacks with Lys15 and Arg19. ( E ) Y17A mutant almost failed to inhibit Cas9-mediated DNA cleavage. This gel is representative of three replicate experiments. ( F ) Y17A mutant significantly impaired the AcrIIA18-induced sgRNA cleavage. This gel is representative of three replicate experiments.

Journal: Nucleic Acids Research

Article Title: Inhibition mechanisms of CRISPR-Cas9 by AcrIIA17 and AcrIIA18

doi: 10.1093/nar/gkab1197

Figure Lengend Snippet: The N-terminal β-hairpin in NTD is critical for AcrIIA18 function. ( A ) The charged residues of AcrIIA18 selected for mutagenesis are shown in pink sphere representations ( B ) In vitro DNA cleavage assay in the presence of the wild-type or mutated AcrIIA18. This gel is representative of three replicate experiments. ( C ) Representative TBE-urea denaturing gels showing the sgRNA cleavage by wild-type and mutated AcrIIA18 in the presence of Cas9. This gel is representative of three replicate experiments. ( D ) The V-shaped groove of AcrIIA18. Charged residues Lys15, Arg19 and Arg52 are located in the V-shaped groove. Tyr17 stacks with Lys15 and Arg19. ( E ) Y17A mutant almost failed to inhibit Cas9-mediated DNA cleavage. This gel is representative of three replicate experiments. ( F ) Y17A mutant significantly impaired the AcrIIA18-induced sgRNA cleavage. This gel is representative of three replicate experiments.

Article Snippet: The cleavage products were separated and visualized by 0.5% TBE agarose gel pertained with StarStain Red Nucleic Acid Dye (GenStar).

Techniques: Mutagenesis, In Vitro, DNA Cleavage Assay

AcrIIA18 triggers sgRNA truncation, and the product is incapable of inducing Cas9-mediated DNA cleavage. ( A ) EMSA was performed using the carboxyfluorescein (FAM)-labeled target DNA as a probe. EMSA showing Cas9 binding to DNA in the presence of either AcrIIA18 or 15 nt sgRNA. 15 nt sgRNA: sgRNA with 15 nt spacer. This gel is representative of three replicate experiments. ( B ) Schematic diagrams of sgRNA. The proposed cleavage site of spacer is indicated by a scissors. ( C ) AcrIIA18 induces the truncation of sgRNA to 15 nt spacer. 15 nt sgRNA and 14 nt sgRNA were used as marker. This gel is representative of three replicate experiments. ( D ) Representative TBE–urea denaturing gels showing sgRNAs of 30, 20 and 15-nt, respectively, cleavage by AcrIIA18. This gel is representative of three replicate experiments. ( E ) In vitro DNA cleavage assay for Cas9 complexed with full-length (20 nt) or 15 nt sgRNA. This gel is representative of three replicate experiments.

Journal: Nucleic Acids Research

Article Title: Inhibition mechanisms of CRISPR-Cas9 by AcrIIA17 and AcrIIA18

doi: 10.1093/nar/gkab1197

Figure Lengend Snippet: AcrIIA18 triggers sgRNA truncation, and the product is incapable of inducing Cas9-mediated DNA cleavage. ( A ) EMSA was performed using the carboxyfluorescein (FAM)-labeled target DNA as a probe. EMSA showing Cas9 binding to DNA in the presence of either AcrIIA18 or 15 nt sgRNA. 15 nt sgRNA: sgRNA with 15 nt spacer. This gel is representative of three replicate experiments. ( B ) Schematic diagrams of sgRNA. The proposed cleavage site of spacer is indicated by a scissors. ( C ) AcrIIA18 induces the truncation of sgRNA to 15 nt spacer. 15 nt sgRNA and 14 nt sgRNA were used as marker. This gel is representative of three replicate experiments. ( D ) Representative TBE–urea denaturing gels showing sgRNAs of 30, 20 and 15-nt, respectively, cleavage by AcrIIA18. This gel is representative of three replicate experiments. ( E ) In vitro DNA cleavage assay for Cas9 complexed with full-length (20 nt) or 15 nt sgRNA. This gel is representative of three replicate experiments.

Article Snippet: The cleavage products were separated and visualized by 0.5% TBE agarose gel pertained with StarStain Red Nucleic Acid Dye (GenStar).

Techniques: Labeling, Binding Assay, Marker, In Vitro, DNA Cleavage Assay